Orexin R1/HCRTR1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95106
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Orexin R1/HCRTR1 Antibody - Azide and BSA Free
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2). HPLLFKSTARRARGSILGIWAVSLAIMVPQAAVMECSSVLPELANRTRLFSVCDERWADDLYPKIYHSCFFIVTYLAPLGLMAMAYFQIFRKLWGRQIPGT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Orexin R1/HCRTR1 Antibody - Azide and BSA Free
![Western Blot: Orexin R1/HCRTR1 AntibodyAzide and BSA Free [NBP2-95106]](https://resources.bio-techne.com/images/products/Orexin-R1-HCRTR1-Antibody-Western-Blot-NBP2-95106-img0001.jpg "Western Blot: Orexin R1/HCRTR1 AntibodyAzide and BSA Free [NBP2-95106]")
Western Blot: Orexin R1/HCRTR1 AntibodyAzide and BSA Free [NBP2-95106]
Western Blot: Orexin R1/HCRTR1 Antibody [NBP2-95106] - Analysis of extracts of C6 cells, using HCRTR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.![Immunohistochemistry: Orexin R1/HCRTR1 Antibody - Azide and BSA Free [NBP2-95106]](https://resources.bio-techne.com/images/products/Orexin-R1-HCRTR1-Antibody-Immunohistochemistry-NBP2-95106-img0002.jpg "Immunohistochemistry: Orexin R1/HCRTR1 Antibody - Azide and BSA Free [NBP2-95106]")
Immunohistochemistry: Orexin R1/HCRTR1 Antibody - Azide and BSA Free [NBP2-95106]
Immunohistochemistry: Orexin R1/HCRTR1 Antibody [NBP2-95106] - Analysis of mouse brain using HCRTR1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Orexin R1/HCRTR1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Orexin R1/HCRTR1
Long Name
Hypocretin Receptor Type 1
Alternate Names
HCRTR1, OrexinR1, OX1R
Gene Symbol
HCRTR1
Additional Orexin R1/HCRTR1 Products
Product Documents for Orexin R1/HCRTR1 Antibody - Azide and BSA Free
Product Specific Notices for Orexin R1/HCRTR1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov