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ORF73/HHV8 Antibody (HHV8/3633R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07279

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HHV8/3633R

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant protein corresponding to the latent nuclear antigen 1 molecule of ORF73/HHV8

Localization

Nuclear (virus in Kaposi's sarcoma)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08397)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ORF73/HHV8 Antibody (HHV8/3633R)

Immunohistochemistry-Paraffin: ORF73/HHV8 Antibody (HHV8/3633R) [NBP3-07279]

Immunohistochemistry-Paraffin: ORF73/HHV8 Antibody (HHV8/3633R) [NBP3-07279]

Immunohistochemistry-Paraffin: ORF73/HHV8 Antibody (HHV8/3633R) [NBP3-07279] - Formalin-fixed, paraffin-embedded human Kaposi's sarcoma stained with ORF73/HHV8 Recombinant Rabbit Monoclonal Antibody (ORF73/HHV8/3633R).

Applications for ORF73/HHV8 Antibody (HHV8/3633R)

Application
Recommended Usage

ELISA

1-5 ug/ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ORF73 HHV8

HHV8 has been found to be associated with three different diseases observed in AIDS patients; kaposi's sarcoma, primary effusion lymphoma (which is a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease. To date there is much evidence to support a direct role for HHV8 in kaposi's sarcoma.

Long Name

ORF73 of Human Herpesvirus 8

Alternate Names

HHV8GK18_gp81, LANA1

Gene Symbol

HHV8gp78

Additional ORF73 HHV8 Products

Product Documents for ORF73/HHV8 Antibody (HHV8/3633R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ORF73/HHV8 Antibody (HHV8/3633R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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