ORF73/HHV8 Antibody (LN53)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07329
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2c Kappa Clone # LN53
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant protein corresponding to the latent nuclear antigen 1 molecule of ORF73/HHV8
Localization
Nuclear (virus in Kaposi's sarcoma)
Clonality
Monoclonal
Host
Rat
Isotype
IgG2c Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08217)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for ORF73/HHV8 Antibody (LN53)
Application
Recommended Usage
ELISA
1-5 ug/ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ORF73 HHV8
Long Name
ORF73 of Human Herpesvirus 8
Alternate Names
HHV8GK18_gp81, LANA1
Gene Symbol
HHV8gp78
Additional ORF73 HHV8 Products
Product Documents for ORF73/HHV8 Antibody (LN53)
Product Specific Notices for ORF73/HHV8 Antibody (LN53)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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