Ornithine Decarboxylase Antibody (ODC1/487)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44735

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-44735-0.02mg
NBP2-44735-0.1mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # ODC1/487

Concentration

0.2 mg/ml

View all ODC1 Antibodies »

Product Specifications

Immunogen

Recombinant human Ornithine Decarboxylase protein (Uniprot: P11926)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47837)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Ornithine Decarboxylase Antibody (ODC1/487)

Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735]

Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735]

Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - IF staining of LNCap cells using AF488 labeled Ornithine Decarboxylase Antibody (ODC1/487). (Green). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735]

Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with Ornithine Decarboxylase Antibody (ODC1/487)
Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735]

Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735]

Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/487) [NBP2-44735] - An intracellular stain was performed on K562 cells with Ornithine Decarboxylase [ODC1/487] Antibody NBP2-47837AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
View 2 more images

Applications for Ornithine Decarboxylase Antibody (ODC1/487)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ODC1

Ornithine Decarboxylase encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter.

Long Name

Ornithine Decarboxylase 1

Alternate Names

ODC

Gene Symbol

ODC1

UniProt

P11926

Additional ODC1 Products

Product Documents for Ornithine Decarboxylase Antibody (ODC1/487)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Ornithine Decarboxylase Antibody (ODC1/487)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.