ORP150/HSP12A Antibody (6H7X2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15331

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15331-100ul
NBP3-15331-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6H7X2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ORP150/HSP12A Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human ORP150/HSP12A (Q9Y4L1). MADKVRRQRPRRRVCWALVAVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPVIVTLKENERFFGDSAASMAIKNPKATLRYFQHLLGKQADNPHVALYQARFPEHELTFDPQRQTVHFQISSQLQFSPEEVL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ORP150/HSP12A Antibody (6H7X2)

Western Blot: ORP150/HSP12A Antibody (6H7X2) [NBP3-15331]

Western Blot: ORP150/HSP12A Antibody (6H7X2) [NBP3-15331]

Western Blot: ORP150/HSP12A Antibody (6H7X2) [NBP3-15331] - Western blot analysis of extracts of various cell lines, using ORP150/HSP12A Rabbit mAb (NBP3-15331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (6H7X2) [NBP3-15331]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (6H7X2) [NBP3-15331]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (6H7X2) [NBP3-15331] - Immunohistochemistry of paraffin-embedded rat pancreas using ORP150/HSP12A Rabbit mAb (NBP3-15331) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for ORP150/HSP12A Antibody (6H7X2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ORP150/HSP12A

HYOU1 is encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Long Name

Oxygen Regulated Protein, 150 kDa

Alternate Names

HSP12A, HYOU1

Gene Symbol

HYOU1

Additional ORP150/HSP12A Products

Product Documents for ORP150/HSP12A Antibody (6H7X2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ORP150/HSP12A Antibody (6H7X2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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