OS9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-519

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all OS9 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of isoform 1 of the human OS9 protein (within residues 300-400). [Swiss-Prot# Q13438]

Specificity

This is specific for isoforms 1 and 2 of human OS-9.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OS9 Antibody

Knockdown Validated: OS9 Antibody [NB100-519]

Knockdown Validated: OS9 Antibody [NB100-519]

Knockdown Validated: OS9 Antibody [NB100-519] - Contribution and interaction of OS-9 for degradation of SHH proteins. Cells stably expressing the N278A-HA precursor were depleted of OS-9 by siRNA, and the fate of N278A was followed after cycloheximide (CHX) addition. All samples were analyzed by SDS-PAGE and immunoblotting with HA antibodies. Controls were treated with a commercial negative control siRNA. The samples were also immunoblotted for OS-9 to show the extent of depletion. Immunoblotting with GAPDH antibodies served as loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092164) licensed under a CC-BY license.
Western Blot: OS9 Antibody [NB100-519]

Western Blot: OS9 Antibody [NB100-519]

Western Blot: OS9 Antibody [NB100-519] - Detection of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2 by western analysis.
Immunohistochemistry: OS9 Antibody [NB100-519]

Immunohistochemistry: OS9 Antibody [NB100-519]

Immunohistochemistry: OS9 Antibody [NB100-519] - Staining of OS9 in mouse smooth muscle.
View 1 more images

Applications for OS9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.2-1.0 ug/ml

Immunohistochemistry

0.5-1.0 ug/ml

Immunohistochemistry-Paraffin

1:100-1:250

Immunoprecipitation

1:10-1:500

Western Blot

0.2-1.0 ug/ml
Application Notes
In WB, bands can be seen at ~75 kDa representing isoform 1 and at ~70 kDa representing isoform 2.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.01% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: OS9

OS9 (protein OS-9 or amplified in osteosarcoma 9) is a lectin/glycoprotein that functions in ER quality control and ER-associated degradation (ERAD pathway) of newly synthesized proteins. TRPV4 is its major target and OS9 also interact with GRP94, DERL2, HSP90B1, CREB3, ERLEC1, HSPA5 etc., forms a part of HRD1 ubiquitin ligase complex (SYVN1/HRD1 and SEL1L) which is involved in retrotranslocation of misfolded proteins from ER to cytosol for ubiquitin dependent degradation. OS9 is essential for recognizition of terminally misfolded non-glycosylated proteins or improperly folded glycoproteins, binding with them for their retention in ER, and transferring them to ubiquitination machinery for degradation. Moreover, it has also been shown to specifically protect some proteins from degradation process before maturation, ensuring their biological functionality. Because OS9 is implicated in protein quality control, it is closely related to ER stress, and gets up-regulated in response to ER stress. Recently, it has been shown to highly express in adipocytes wherein it regulated lipid storage, and its overexpression is also observed in sarcomas especially in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines.

Alternate Names

Amplified in osteosarcoma 9, endoplasmic reticulum lectin 2, ERLEC2, erlectin 2, OS-9, osteosarcoma amplified 9, endoplasmic reticulum associated protein, osteosarcoma amplified 9, endoplasmic reticulum lectin, protein OS-9

Entrez Gene IDs

10956 (Human); 216440 (Mouse)

Gene Symbol

OS9

UniProt

Q13438 (Human)

Additional OS9 Products

Product Documents for OS9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for OS9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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