OSCAR Antibody (5B8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11201

Novus Biologicals, part of Bio-Techne
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NBP3-11201
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Functional

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 5B8

Concentration

0.1 mg/ml

View all OSCAR Antibodies »

Product Specifications

Immunogen

The monoclonal antibody 5B8 reacts with mouse osteoclast-associated receptor (OSCAR).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<24 EU/mg

Scientific Data Images for OSCAR Antibody (5B8)

Flow Cytometry: OSCAR Antibody (5B8) [NBP3-11201]

Flow Cytometry: OSCAR Antibody (5B8) [NBP3-11201]

Flow Cytometry: OSCAR Antibody (5B8) [NBP3-11201] - Analysis of OSCAR in RAW cells.

Applications for OSCAR Antibody (5B8)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The monoclonal antibody can be used for analysis of mouse OSCAR in flow cytometry as well as functional studies for osteoclastogenesis. For Flow Cytometry and Immunocytochemistry/Immunofluorescence, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: OSCAR

The monoclonal antibody 5B8 reacts with mouse osteoclast-associated receptor (OSCAR), which is a 265 amino acid type 1 transmembrane protein belonging to the leukocyte receptor complex protein family that play critical roles in the regulation of both innate and adaptive immune responses. In mice, OSCAR is a RANKL-inducible gene which- is expressed during the later stages of pre-osteoclast maturation and is shown to be a potent FcR and gamma-associated costimulatory receptor in vitro. OSCAR promotes osteoclastogenesis in vivo, and OSCAR binding to its collagen ligand led to signaling that- is involved in functional differentiation of leukocytes. In recent studies human OSCAR has been shown to play multiple roles in the immune system including activation and maturation of immune cells, prevention of apoptosis and enhancement of pro-inflammatory circuits. The latter by- inducing intracellular calcium release resulting in sustained IL-8 and chemokine expression leading to chemotaxis of Th2 effector and regulatory T-cells. In a- murine model using calvarias bone explants, the synovial fluid of osteoarthritis patients resulted in increased OSCAR mRNA expression suggesting that OSCAR might contribute to in the degenerative process. Moreover, in a recent study- OSCAR was found to be- induced in monocytes of RA patients, facilitating their differentiation into osteoclasts and bone resorption. The monoclonal antibody, 5B8, can be used for analysis of mouse OSCAR in flow-cytometry as well as functional studies for osteoclastogenesis.

Long Name

Osteoclast-associated Receptor

Alternate Names

hOSCAR, MGC33613, osteoclast associated receptor OSCAR-S1, osteoclast associated receptor OSCAR-S2, osteoclast associated, immunoglobulin-like receptor, osteoclast-associated immunoglobulin-like receptor, Osteoclast-associated receptor, PIGR3, PIgR-3, Poly-Ig receptor 3, Polymeric immunoglobulin receptor 3

Gene Symbol

OSCAR

Additional OSCAR Products

Product Documents for OSCAR Antibody (5B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OSCAR Antibody (5B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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