OSGEP Antibody (OTI9E3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73163

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI9E3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all OSGEP Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-335 of human OSGEP(NP_060277) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

36.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OSGEP Antibody (OTI9E3) - Azide and BSA Free

Western Blot: OSGEP Antibody (OTI9E3)Azide and BSA Free [NBP2-73163]

Western Blot: OSGEP Antibody (OTI9E3)Azide and BSA Free [NBP2-73163]

Western Blot: OSGEP Antibody (OTI9E3) - Azide and BSA Free [NBP2-73163] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSGEP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSGEP.
Immunohistochemistry: OSGEP Antibody (OTI9E3) - Azide and BSA Free [NBP2-73163]

Immunohistochemistry: OSGEP Antibody (OTI9E3) - Azide and BSA Free [NBP2-73163]

Immunohistochemistry: OSGEP Antibody (OTI9E3) - Azide and BSA Free [NBP2-73163] - Staining of paraffin-embedded Human Kidney tissue using anti-OSGEP mouse monoclonal antibody.
Flow Cytometry: OSGEP Antibody (OTI9E3) - Azide and BSA Free [NBP2-73163]

Flow Cytometry: OSGEP Antibody (OTI9E3) - Azide and BSA Free [NBP2-73163]

Flow Cytometry: OSGEP Antibody (OTI9E3) - Azide and BSA Free [NBP2-73163] - Analysis of Hela cells, using anti-OSGEP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications for OSGEP Antibody (OTI9E3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OSGEP

O-sialoglycoprotein endopeptidases specifically cleave the polypeptide backbone of membrane glycoproteins that contain clusters of O-linked sialoglycans

Alternate Names

EC 3.4.24.57, GCPL1FLJ20411, hOSGEP, KAE1, OSGEP1, O-sialoglycoprotein endopeptidase, probable O-sialoglycoprotein endopeptidase, PRSMG1, t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEP

Gene Symbol

OSGEP

Additional OSGEP Products

Product Documents for OSGEP Antibody (OTI9E3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OSGEP Antibody (OTI9E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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