Osteopontin/OPN Antibody (7C5H12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37423

Novus Biologicals, part of Bio-Techne
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NBP2-37423
NBP2-37423-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7C5H12

Format

BSA Free

Concentration

1 mg/ml

View all Osteopontin/OPN Antibodies »

Product Summary for Osteopontin/OPN Antibody (7C5H12) - BSA Free

Immunogen

Purified recombinant fragment of human Osteopontin/OPN (AA: 167-314) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Osteopontin/OPN Antibody (7C5H12) - BSA Free

Western Blot: Osteopontin/OPN Antibody (7C5H12)BSA Free [NBP2-37423]

Western Blot: Osteopontin/OPN Antibody (7C5H12)BSA Free [NBP2-37423]

Western Blot: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis using SPP1 mAb against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (7C5H12) - BSA Free [NBP2-37423]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (7C5H12) - BSA Free [NBP2-37423]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis of endometrial cancer tissues using SPP1 mouse mAb with DAB staining.
Western Blot: Osteopontin/OPN Antibody (7C5H12)BSA Free [NBP2-37423]

Western Blot: Osteopontin/OPN Antibody (7C5H12)BSA Free [NBP2-37423]

Western Blot: Osteopontin/OPN Antibody (7C5H12) [NBP2-37423] - Analysis using SPP1 mAb against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)
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Applications for Osteopontin/OPN Antibody (7C5H12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (7C5H12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Osteopontin/OPN Antibody (7C5H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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