Osteopontin/OPN Antibody (rOSP/8856) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24112

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # rOSP/8856

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Osteopontin/OPN Antibodies »

Product Specifications

Immunogen

Recombinant full-length rat Osteopontin/OPN protein

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for Osteopontin/OPN Antibody (rOSP/8856) - Azide and BSA Free

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (rOSP/8856) - Azide and BSA Free [NBP3-24112] -

Formalin-fixed, paraffin-embedded human prostate stained with Osteopontin/OPN antibody (rOSP/8856) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for Osteopontin/OPN Antibody (rOSP/8856) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (rOSP/8856) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Osteopontin/OPN Antibody (rOSP/8856) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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