Osteopontin/OPN Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59190
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to SPP1 (secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)) The peptide sequence was selected from the C terminal of SPP1 (NP_000573). Peptide sequence DSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASS The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for Osteopontin/OPN Antibody
![Western Blot: Osteopontin/OPN Antibody [NBP1-59190]](https://resources.bio-techne.com/images/products/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0002.jpg "Western Blot: Osteopontin/OPN Antibody [NBP1-59190]")
Western Blot: Osteopontin/OPN Antibody [NBP1-59190]
Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190]](https://resources.bio-techne.com/images/products/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-59190-img0003.jpg "Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190]")
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190]
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.![Western Blot: Osteopontin/OPN Antibody [NBP1-59190]](https://resources.bio-techne.com/images/products/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0004.jpg "Western Blot: Osteopontin/OPN Antibody [NBP1-59190]")
Western Blot: Osteopontin/OPN Antibody [NBP1-59190]
Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%Applications for Osteopontin/OPN Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
4-8 ug/ml
Western Blot
1.0 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26770207).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Osteopontin/OPN
Long Name
Secreted Phosphoprotein 1 [BNSP]
Alternate Names
Eta-1, OPN, Spp1
Entrez Gene IDs
6696 (Human)
Gene Symbol
SPP1
UniProt
Additional Osteopontin/OPN Products
Product Documents for Osteopontin/OPN Antibody
Product Specific Notices for Osteopontin/OPN Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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