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Otubain-2 Antibody (139)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90191

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-90191-100ul
NBP2-90191-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 139

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Otubain-2 (Uniprot#: Q96DC9-1; Met1-His234).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Otubain-2 Antibody (139)

Immunocytochemistry/ Immunofluorescence: Otubain-2 Antibody (139) [NBP2-90191]

Immunocytochemistry/ Immunofluorescence: Otubain-2 Antibody (139) [NBP2-90191]

Immunocytochemistry/Immunofluorescence: Otubain-2 Antibody (139) [NBP2-90191] - Immunofluorescence staining of Human Otubain-2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Otubain-2 monoclonal antibody (1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(TM) 549-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.

Applications for Otubain-2 Antibody (139)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otubain-2

Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 2

Alternate Names

C14orf137, OTU2, OTUB2, Otubain2

Gene Symbol

OTUB2

Additional Otubain-2 Products

Product Documents for Otubain-2 Antibody (139)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Otubain-2 Antibody (139)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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