Otubain-2 Antibody (2E7)
Novus Biologicals, part of Bio-Techne | Catalog # H00078990-M14
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunoprecipitation, Sandwich ELISA
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2E7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
OTUB2 (NP_075601, 126 a.a. ~ 234 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH
Specificity
OTUB2 - OTU domain, ubiquitin aldehyde binding 2 (2E7)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Otubain-2 Antibody (2E7)
Sandwich ELISA: Otubain-2 Antibody (2E7) [H00078990-M14] - Detection limit for recombinant GST tagged OTUB2 is 1 ng/ml as a capture antibody.
![Immunoprecipitation: Otubain-2 Antibody (2E7) [H00078990-M14]](https://resources.bio-techne.com/images/products/Otubain-2-Antibody-2E7-Immunoprecipitation-H00078990-M14-img0001.jpg "Immunoprecipitation: Otubain-2 Antibody (2E7) [H00078990-M14]")
Immunoprecipitation: Otubain-2 Antibody (2E7) [H00078990-M14]
Immunoprecipitation: Otubain-2 Antibody (2E7) [H00078990-M14] - Analysis of OTUB2 transfected lysate using anti-OTUB2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OTUB2 rabbit polyclonal antibody.Applications for Otubain-2 Antibody (2E7)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for ELISA.WB was previously validated through publication (30241937) for a previous lot. Current lot has been reported by several customers to not work in WB and is not recommended.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Otubain-2
Long Name
OTU Domain, Ubiquitin Aldehyde Binding 2
Alternate Names
C14orf137, OTU2, OTUB2, Otubain2
Entrez Gene IDs
78990 (Human)
Gene Symbol
OTUB2
UniProt
Additional Otubain-2 Products
Product Documents for Otubain-2 Antibody (2E7)
Product Specific Notices for Otubain-2 Antibody (2E7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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