Otubain-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88410

Novus Biologicals, part of Bio-Techne
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NBP1-88410
NBP1-88410-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Otubain-2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNIL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Otubain-2 Antibody

Immunohistochemistry-Paraffin: Otubain-2 Antibody [NBP1-88410]

Immunohistochemistry-Paraffin: Otubain-2 Antibody [NBP1-88410]

Immunohistochemistry-Paraffin: Otubain-2 Antibody [NBP1-88410] - Staining in human testis and skeletal muscle tissues using anti-OTUB2 antibody. Corresponding OTUB2 RNA-seq data are presented for the same tissues.
Western Blot: Otubain-2 Antibody [NBP1-88410]

Western Blot: Otubain-2 Antibody [NBP1-88410]

Western Blot: Otubain-2 Antibody [NBP1-88410] - Analysis in control (vector only transfected HEK293T lysate) and OTUB2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Otubain-2 Antibody [NBP1-88410]

Immunohistochemistry-Paraffin: Otubain-2 Antibody [NBP1-88410]

Immunohistochemistry-Paraffin: Otubain-2 Antibody [NBP1-88410] - Staining of human testis shows distinct nuclear positivity in seminiferus duct cells.
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Applications for Otubain-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Otubain-2

Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 2

Alternate Names

C14orf137, OTU2, OTUB2, Otubain2

Gene Symbol

OTUB2

Additional Otubain-2 Products

Product Documents for Otubain-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Otubain-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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