OTUD6B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85653

Novus Biologicals, part of Bio-Techne
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NBP1-85653
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all OTUD6B Antibodies »

Product Summary for OTUD6B Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IQGMKNAVPKNDKKRRKQLTEDVAKLEKEMEQKHREELEQLKLTTKENKIDSVAVNISNLV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OTUD6B Antibody

Western Blot: OTUD6B Antibody [NBP1-85653]

Western Blot: OTUD6B Antibody [NBP1-85653]

Western Blot: OTUD6B Antibody [NBP1-85653] - Analysis using Anti-OTUD6B antibody NBP1-85653 (A) shows similar pattern to independent antibody NBP1-85652 (B).
Immunocytochemistry/ Immunofluorescence: OTUD6B Antibody [NBP1-85653]

Immunocytochemistry/ Immunofluorescence: OTUD6B Antibody [NBP1-85653]

Immunocytochemistry/Immunofluorescence: OTUD6B Antibody [NBP1-85653] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.
Immunohistochemistry-Paraffin: OTUD6B Antibody [NBP1-85653]

Immunohistochemistry-Paraffin: OTUD6B Antibody [NBP1-85653]

Immunohistochemistry-Paraffin: OTUD6B Antibody [NBP1-85653] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and glomeruli.

Applications for OTUD6B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OTUD6B

Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA5 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.[supplied by OMIM]

Alternate Names

CGI-77, DUBA5DUBA-5, OTU domain containing 6B, OTU domain-containing protein 6B

Gene Symbol

OTUD6B

Additional OTUD6B Products

Product Documents for OTUD6B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OTUD6B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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