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Otx2 Antibody (PCRP-OTX2-1E10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13986

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-13986-100ug
NBP3-13986-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-OTX2-1E10

Concentration

0.2 mg/ml

Product Summary for Otx2 Antibody (PCRP-OTX2-1E10)

Immunogen

Recombinant human full-lengthOtx2protein (Uniprot: P32243)

Reactivity Notes

Predicted to react in Chick, Mouse, Rat, Xenopus and Zebrafish.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

71.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14028)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Applications for Otx2 Antibody (PCRP-OTX2-1E10)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Otx2

Homeobox protein Otx2 is a member of the bicoid sub-family of homeodomain-containing transcription factors. It is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sense organs. Two distinct isoforms have been identified. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Gene Symbol

OTX2

UniProt

Additional Otx2 Products

Product Documents for Otx2 Antibody (PCRP-OTX2-1E10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Otx2 Antibody (PCRP-OTX2-1E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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