Oxytocin/Neurophysin I Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76346
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Oxytocin/Neurophysin I Antibody
Immunogen
Peptide with sequence DGCRTDPACDPES corresponding to C-Terminus (near) according to Uniprot Mouse NP_035155.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Oxytocin/Neurophysin I Antibody
![Flow Cytometry: Oxytocin/Neurophysin I Antibody [NBP2-76346]](https://resources.bio-techne.com/images/products/Oxytocin-Antibody-Flow-Cytometry-NBP2-76346-img0002.jpg "Flow Cytometry: Oxytocin/Neurophysin I Antibody [NBP2-76346]")
Flow Cytometry: Oxytocin/Neurophysin I Antibody [NBP2-76346]
Flow Cytometry: Oxytocin/Neurophysin I Antibody [NBP2-76346] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.![Immunofluorescence: Oxytocin/Neurophysin I Antibody [NBP2-76346]](https://resources.bio-techne.com/images/products/Oxytocin-Antibody-Immunofluorescence-NBP2-76346-img0001.jpg "Immunofluorescence: Oxytocin/Neurophysin I Antibody [NBP2-76346]")
Immunofluorescence: Oxytocin/Neurophysin I Antibody [NBP2-76346]
Immunofluorescence: Oxytocin/Neurophysin I Antibody [NBP2-76346] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for Oxytocin/Neurophysin I Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Peptide ELISA
Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 22 kDa band in mouse liver and lung lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 12.9 kDa band according to NP_035155.1. The 22 kDa band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Oxytocin/Neurophysin I
Alternate Names
neurophysin I, OT, OT-NPI, OXT, OXT-NPI, oxytocin, prepro- (neurophysin I), oxytocin, prepropeptide, oxytocin-neurophysin 1, oxytocin-neurophysin I, preproprotein
Gene Symbol
OXT
Additional Oxytocin/Neurophysin I Products
Product Documents for Oxytocin/Neurophysin I Antibody
Product Specific Notices for Oxytocin/Neurophysin I Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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