P-Selectin/CD62P Antibody (Psel.KO.2.7) [mFluor Violet 500 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65392MFV500

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # Psel.KO.2.7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all P-Selectin/CD62P Antibodies »

Product Specifications

Immunogen

P-selectin transfected 300.19 cells

Reactivity Notes

Predicted cross-reactivities: Mouse, Equine, Goat, Bovine, Feline, Sheep, Rat

Localization

Membrane; single pass type I membrane protein.

Specificity

NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for P-Selectin/CD62P Antibody (Psel.KO.2.7) [mFluor Violet 500 SE]

P-Selectin/CD62P Antibody (Psel.KO.2.7) [mFluor Violet 500 SE] -

P-Selectin/CD62P Antibody (Psel.KO.2.7) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.

Applications for P-Selectin/CD62P Antibody (Psel.KO.2.7) [mFluor Violet 500 SE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: P-Selectin/CD62P

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Gene Symbol

SELP

Additional P-Selectin/CD62P Products

Product Documents for P-Selectin/CD62P Antibody (Psel.KO.2.7) [mFluor Violet 500 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for P-Selectin/CD62P Antibody (Psel.KO.2.7) [mFluor Violet 500 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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