p107 Antibody

Immunocytochemistry/ Immunofluorescence: p107 Antibody [NBP2-33735]

Immunocytochemistry/Immunofluorescence: p107 Antibody [NBP2-33735] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735]

Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human testis shows moderate nuclear postivity in cells in seminiferous ducts and Leydig cells.

Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735]

Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human colon shows moderate nuclear positivity in glanular cells.

Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735]

Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human kidney shows moderate nuclear postivity in cells in tubules.

Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735]

Immunohistochemistry-Paraffin: p107 Antibody [NBP2-33735] - Staining of human liver shows no positivity in hepatocytes as expected.

Western Blot: p107 Antibody [NBP2-33735] -

Western Blot: p107 Antibody [NBP2-33735] - Selinexor alters pocket protein expression in nuclear & cytoplasmic compartments(A) Representative images of p107, p130 & RB (green) IF staining of bladder cancer cells treated with vehicle (V) or selinexor (S) for 48 hours. Tubulin staining (red) & DAPI staining (blue) served to define the cytoplasmic & nuclear compartments, respectively. The inserts are magnifications of the boxed cells. (B) Quantification of staining intensity of pocket proteins normalized to DAPI. (C) Nuclear & cytoplasmic fractions of cell treated with vehicle or 0.15 uM selinexor (UM-UC-3 & T24 cells), 0.25 uM selinexor (J82) & 0.5 uM selinexor (TCCSUP) for 72 hours were assessed for the expression of RB, p107 & p130. Nup62 & tubulin were used as markers for the nuclear & cytoplasmic fractions, respectively. (D) T24 & UM-UC-3 cells transfected with siC or siRB & were treated with vehicle or 0.1 uM selinexor for 72 hours. The results are shown as percent cell viability comparing drug treated to vehicle treated cells. (E) Palbociclib reduces T24 & UM-UC-3 bladder tumor cells viability in a dose dependent manner. (F) Combined selinexor (0.1 uM) & palbociclib (0.5 uM) treatment is more effective in reducing viability of cells than either treatment alone where the CI = 1.04 for UM-UC-3 cells & 1.02 for T24 cells indicating an additive response. Error bars = ± standard deviation. Student’s t test; * denotes p ≤ 0.05, ** denotes p ≤ 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30349650), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: p107 Antibody [NBP2-33735] -

Immunocytochemistry/ Immunofluorescence: p107 Antibody [NBP2-33735] - Selinexor alters pocket protein expression in nuclear & cytoplasmic compartments(A) Representative images of p107, p130 & RB (green) IF staining of bladder cancer cells treated with vehicle (V) or selinexor (S) for 48 hours. Tubulin staining (red) & DAPI staining (blue) served to define the cytoplasmic & nuclear compartments, respectively. The inserts are magnifications of the boxed cells. (B) Quantification of staining intensity of pocket proteins normalized to DAPI. (C) Nuclear & cytoplasmic fractions of cell treated with vehicle or 0.15 uM selinexor (UM-UC-3 & T24 cells), 0.25 uM selinexor (J82) & 0.5 uM selinexor (TCCSUP) for 72 hours were assessed for the expression of RB, p107 & p130. Nup62 & tubulin were used as markers for the nuclear & cytoplasmic fractions, respectively. (D) T24 & UM-UC-3 cells transfected with siC or siRB & were treated with vehicle or 0.1 uM selinexor for 72 hours. The results are shown as percent cell viability comparing drug treated to vehicle treated cells. (E) Palbociclib reduces T24 & UM-UC-3 bladder tumor cells viability in a dose dependent manner. (F) Combined selinexor (0.1 uM) & palbociclib (0.5 uM) treatment is more effective in reducing viability of cells than either treatment alone where the CI = 1.04 for UM-UC-3 cells & 1.02 for T24 cells indicating an additive response. Error bars = ± standard deviation. Student’s t test; * denotes p ≤ 0.05, ** denotes p ≤ 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30349650), licensed under a CC-BY license. Not internally tested by Novus Biologicals.