p120-catenin [p Tyr228] Antibody (M356)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23203

Novus Biologicals, part of Bio-Techne
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NBP3-23203
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M356

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p120-catenin Antibodies »

Product Summary for p120-catenin [p Tyr228] Antibody (M356)

Immunogen

Clone M356 was generated from a phospho-p120-catenin (Tyr-228) synthetic peptide containing amino acid residues around tyrosine 228 of human p120-catenin. This peptide sequence is highly conserved in rat and mouse p120-catenin. [UniProt# O60716]

Modification

p Tyr228

Specificity

The antibody detects a 110 kDa protein corresponding to the molecular mass of p120-catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells treated with pervanadate. This antibody does not detect this band after alkaline phosphatase treatment.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for p120-catenin [p Tyr228] Antibody (M356)

p120-catenin [p Tyr228] Antibody (M356)

Immunocytochemistry/Immunofluorescence: p120-catenin [p Tyr228] Antibody (M356) [NBP3-23203] -

Immunocytochemistry/Immunofluorescence: p120-catenin [p Tyr228] Antibody (M356) [NBP3-23203] - Immunocytochemical labeling of p120-catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal p120-catenin (a.a. 275-285), p120-catenin (Tyr-228), p120-catenin (Tyr-280), or p120-catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.
p120-catenin [p Tyr228] Antibody (M356)

Western Blot: p120-catenin [p Tyr228] Antibody (M356) [NBP3-23203] -

Western Blot: p120-catenin [p Tyr228] Antibody (M356) [NBP3-23203] - Western blot analysis of p120-catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-p120-catenin (a.a. 275-285) (lanes 1 & 2), anti-p120-catenin (Tyr-96) (lanes 3 & 4), anti-p120-catenin (Tyr-228) (lanes 5 & 6), anti-p120-catenin (Tyr-280) (lanes 7 & 8) or anti-p120-catenin (Tyr-302) (lanes 9 & 10).

Applications for p120-catenin [p Tyr228] Antibody (M356)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: p120-catenin

The alpha, beta, delta, and gamma -catenins are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. alpha-catenin, a 102 kDa protein, interacts with E-cadherin associated protein, and also associates with other members of the cadherin family, such as N-cadherin and P-cadherin. The 92 kDa beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also complexes with the tumor suppressor protein APC. delta-catenin interacts with Presenilin 1 and is expressed in the brain. The gene encoding delta-catenin maps to human chromosome 5p15.2. A hemizygous loss of the gene encoding delta-catenin leads to the mental retardation associated with Cri-du-Chat syndrome. gamma-catenin, also known as plakoglobin, is an 80-88 kDa protein that binds alpha-catenin and N-cadherin. In addition, the transmembrane phosphatase PTPm associates with catenin/cadherin complexes and may regulate complex signaling.

Alternate Names

Cadherin-associated Src substrate, CAS, catenin (cadherin-associated protein), delta 1, catenin delta-1, CTNND, KIAA0384P120CAS, p120, p120 catenin, p120(CAS), p120(CTN), p120cas, p120ctn

Gene Symbol

CTNND1

Additional p120-catenin Products

Product Documents for p120-catenin [p Tyr228] Antibody (M356)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p120-catenin [p Tyr228] Antibody (M356)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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