p130 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15468

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p130 Antibodies »

Product Summary for p130 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 413-613 of human p130 (NP_005602.3). SPCVTPVSTATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDFSNCAKEIASKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVVTFSYKPPGNFPFITEIFDVPLYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKPESPLWEKIRDNE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p130 Antibody - Azide and BSA Free

Western Blot: p130 AntibodyAzide and BSA Free [NBP3-15468]

Western Blot: p130 AntibodyAzide and BSA Free [NBP3-15468]

Western Blot: p130 Antibody [NBP3-15468] - Western blot analysis of extracts of Neuro-2a cells, using p130 antibody (NBP3-15468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Immunocytochemistry/ Immunofluorescence: p130 Antibody - Azide and BSA Free [NBP3-15468]

Immunocytochemistry/ Immunofluorescence: p130 Antibody - Azide and BSA Free [NBP3-15468]

Immunocytochemistry/Immunofluorescence: p130 Antibody [NBP3-15468] - Immunofluorescence analysis of U2OS cells using p130 Rabbit pAb (NBP3-15468) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Applications for p130 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p130

Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.

Alternate Names

FLJ26459, p130, PRB2, Rb2, RBR-2, retinoblastoma-like 2 (p130), retinoblastoma-like protein 2,130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2

Gene Symbol

RBL2

Additional p130 Products

Product Documents for p130 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p130 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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