p130Cas/BCAR1 Antibody (JE36-56)

Western Blot: p130Cas/BCAR1 Antibody (JE36-56) [NBP3-32071]

Western Blot: p130Cas/BCAR1 Antibody (JE36-56) [NBP3-32071] - Western blot analysis of p130Cas/BCAR1 on different lysates with Rabbit anti-p130Cas/BCAR1 antibody (NBP3-32071) at 1/1,000 dilution.

Lane 1: HUVEC cell lysate
Lane 2: HeLa cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 93 kDa
Observed band size: 110 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32071) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.