p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32750
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE47-97
Concentration
1 mg/ml
Product Summary for p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr410 of Human p130Cas/BCAR1. (Uniprot: P56945)
Modification
p Tyr 410
Localization
Cell junction, focal adhesion, Cytoplasm, Cell projection, axon.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97)
![p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97)](https://resources.bio-techne.com/images/products/nbp3-32750_rabbit-p130cas-bcar1-p-tyr410-mab-je47-97-278202415295539.jpg "Western Blot: p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97) [NBP3-32750]")
Western Blot: p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97) [NBP3-32750]
Western Blot: p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97) [NBP3-32750] - Western blot analysis of p130Cas/BCAR1 on different lysates with Rabbit anti-p130Cas/BCAR1 antibody (NBP3-32750) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 93 kDa
Observed band size: 130 kDa
Exposure time: 1 minute; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32750) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: p130Cas
Long Name
Breast Cancer Anti-estrogen Resistance 1
Alternate Names
BCAR1, CASS1, CRKAS
Gene Symbol
BCAR1
Additional p130Cas Products
Product Documents for p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97)
Product Specific Notices for p130Cas/BCAR1 [p Tyr410] Antibody (JE47-97)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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