p130Cas/BCAR1 Antibody (OTI1A9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00532

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A9

Concentration

0.53 mg/ml

View all p130Cas Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BCAR1 (NP_055382) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

93.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p130Cas/BCAR1 Antibody (OTI1A9)

Western Blot: BCAR1 Antibody (1A9) [NBP2-00532] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: p130Cas/BCAR1 Antibody (OTI1A9) [NBP2-00532]

Immunocytochemistry/ Immunofluorescence: p130Cas/BCAR1 Antibody (OTI1A9) [NBP2-00532]

Immunocytochemistry/Immunofluorescence: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.
Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (OTI1A9) [NBP2-00532]

Immunohistochemistry-Paraffin: p130Cas/BCAR1 Antibody (OTI1A9) [NBP2-00532]

Immunohistochemistry-Paraffin: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody.
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Applications for p130Cas/BCAR1 Antibody (OTI1A9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.53 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p130Cas

BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM].

Long Name

Breast Cancer Anti-estrogen Resistance 1

Alternate Names

BCAR1, CASS1, CRKAS

Entrez Gene IDs

9564 (Human)

Gene Symbol

BCAR1

Additional p130Cas Products

Product Documents for p130Cas/BCAR1 Antibody (OTI1A9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p130Cas/BCAR1 Antibody (OTI1A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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