p130Cas/BCAR1 [p Tyr165] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05456

Novus Biologicals, part of Bio-Techne
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NBP3-05456
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p130Cas Antibodies »

Product Summary for p130Cas/BCAR1 [p Tyr165] Antibody - Azide and BSA Free

Immunogen

A synthetic phosphorylated peptide around Y165 of human p130Cas/BCAR1 (NP_055382.2). DLYQV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p130Cas/BCAR1 [p Tyr165] Antibody - Azide and BSA Free

Western Blot: p130Cas/BCAR1 [p Tyr165] AntibodyAzide and BSA Free [NBP3-05456]

Western Blot: p130Cas/BCAR1 [p Tyr165] AntibodyAzide and BSA Free [NBP3-05456]

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-05456] - Western blot analysis of extracts of PC-12 cells, using p130Cas/BCAR1 antibody (NBP3-05456) at 1:1000 dilution. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37C for 1 hour . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for p130Cas/BCAR1 [p Tyr165] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p130Cas

BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells. Alternate names for BCAR1/p130CAS include breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, CAS scaffolding protein family member 1, CAS, and CRKAS.

Long Name

Breast Cancer Anti-estrogen Resistance 1

Alternate Names

BCAR1, CASS1, CRKAS

Gene Symbol

BCAR1

Additional p130Cas Products

Product Documents for p130Cas/BCAR1 [p Tyr165] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p130Cas/BCAR1 [p Tyr165] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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