p130Cas/BCAR1 [p Tyr751] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23254
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Phospho-p130Cas/BCAR1 (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130Cas/BCAR1. This rat sequence is the same as Tyr-653 in human p130Cas/BCAR1s and Tyr-657 in mouse p130Cas/BCAR1. [UniProt# Q63767]
Modification
p Tyr751
Specificity
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130Cas/BCAR1 (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier). The antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for p130Cas/BCAR1 [p Tyr751] Antibody
Western Blot: p130Cas/BCAR1 [p Tyr751] Antibody [NBP3-23254] -
Western Blot: p130Cas/BCAR1 [p Tyr751] Antibody [NBP3-23254] - Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130Cas/BCAR1 (NBP3-23253; lanes 1 & 2), anti-p130Cas/BCAR1 (Tyr-751) (NBP3-23254; lanes 3 & 4) or anti-p130Cas/BCAR1 (Tyr-762) (NBP3-23281; lanes 5 & 6).Applications for p130Cas/BCAR1 [p Tyr751] Antibody
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: p130Cas
Long Name
Breast Cancer Anti-estrogen Resistance 1
Alternate Names
BCAR1, CASS1, CRKAS
Gene Symbol
BCAR1
Additional p130Cas Products
Product Documents for p130Cas/BCAR1 [p Tyr751] Antibody
Product Specific Notices for p130Cas/BCAR1 [p Tyr751] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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