p14ARF/CDKN2A Antibody (4C6/4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07234

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C6/4

Concentration

0.2 mg/ml

View all p14ARF/CDKN2A Antibodies »

Product Summary for p14ARF/CDKN2A Antibody (4C6/4)

Immunogen

Recombinant full-length human p14ARF/CDKN2A protein (Uniprot: P42771)

Localization

Nuclear and Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08233)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for p14ARF/CDKN2A Antibody (4C6/4)

Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody (4C6/4) [NBP3-07234]

Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody (4C6/4) [NBP3-07234]

Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody (4C6/4) [NBP3-07234] - Formalin-fixed, paraffin-embedded human Brain stained with p14ARF/CDKN2A Mouse Monoclonal Antibody (4C6/4).
Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody (4C6/4) [NBP3-07234]

Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody (4C6/4) [NBP3-07234]

Immunohistochemistry-Paraffin: p14ARF/CDKN2A Antibody (4C6/4) [NBP3-07234] - Formalin-fixed, paraffin-embedded rat Brain stained with p14ARF/CDKN2A Mouse Monoclonal Antibody (4C6/4).

Applications for p14ARF/CDKN2A Antibody (4C6/4)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: p14ARF/CDKN2A

P14ARF is an alternate reading frame (ARF) product of the CDKN2A locus, which acts as a tumor suppressor by inducing cell cycle arrest in G1 and G2 phases and apoptosis.

The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded by differential splicing of alternative first exons. The p16INK4a (exon 1 alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF protein complexes with MDM2 within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.

Alternate Names

ARF, CDK4 inhibitor p16-INK4, CDK4IP14ARF, CDKN2, cell cycle negative regulator beta, CMM2P16-INK4A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4), INK4, INK4A, MLMP16INK4, MTS-1, MTS1P14, Multiple tumor suppressor 1, p14, p14ARF, P16, p16-INK4, P16INK4A, p16-INK4a, p19, P19ARF, TP16

Gene Symbol

CDKN2A

UniProt

P42771

Additional p14ARF/CDKN2A Products

Product Documents for p14ARF/CDKN2A Antibody (4C6/4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p14ARF/CDKN2A Antibody (4C6/4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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