p16INK4a/CDKN2A Antibody (0D0C8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15420

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15420-100ul
NBP3-15420-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0D0C8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p16INK4a / CDKN2A Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 57-156 of human p16INK4a/CDKN2A (P42771). ARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p16INK4a/CDKN2A Antibody (0D0C8)

Western Blot: p16INK4a/CDKN2A Antibody (0D0C8) [NBP3-15420]

Western Blot: p16INK4a/CDKN2A Antibody (0D0C8) [NBP3-15420]

Western Blot: p16INK4a/CDKN2A Antibody (0D0C8) [NBP3-15420] - Analysis of extracts of HeLa cells, using p16INK4a/CDKN2A Rabbit mAb (NBP3-15420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (0D0C8) [NBP3-15420]

Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (0D0C8) [NBP3-15420]

Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (0D0C8) [NBP3-15420] - Immunofluorescence analysis of HeLa cells using p16INK4a/CDKN2A Rabbit mAb (NBP3-15420) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (0D0C8) [NBP3-15420]

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (0D0C8) [NBP3-15420]

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (0D0C8) [NBP3-15420] - Human cervix cancer using [KO Validated] p16INK4a/CDKN2A Rabbit mAb (NBP3-15420) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for p16INK4a/CDKN2A Antibody (0D0C8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p16INK4a / CDKN2A

CDKN2A/p16INK4a is a gene that codes for a widely expressed protein with four isoforms, measuring 156, 105, 116, and 167 amino acids in length with weights of approximately 17, 11, 12, and 18 kDa respectively. CDKN2A/p16INK4a functions as a negative regulator of the proliferation of cells, which then allows them to interact with cyclins D and to phosphorylate the retinoblastoma protein. Current studies are being done on several diseases and disorders related to this gene including Li-Fraumeni syndrome, malignant peripheral nerve sheath tumor, blastic plasmacytoid dendritic cell, acth-secreting pituitary adenoma, recessive dystrophic epidermolysis bullosa, marginal zone b-cell lymphoma, adult astrocytic tumor, melanoma, and squamous cell carcinoma. CDKN2A/p16INK4a has also been shown to have interactions with MDM2, ZNF420, EGR1, MYCN, and CDK6 in pathways such as the cell cycle, p53 signaling, mitochondrial apoptosis, HIF-1 Alpha, CRHR, pancreatic cancer, and glioma pathways.

Long Name

p16 Cyclin Dependent Kinase 4 Inhibitor 2A

Alternate Names

ARF, CDK4I, CDKN2A, INK4a, MLM, p14ARF, p16, p19ARF

Gene Symbol

CDKN2A

Additional p16INK4a / CDKN2A Products

Product Documents for p16INK4a/CDKN2A Antibody (0D0C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p16INK4a/CDKN2A Antibody (0D0C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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