p16INK4a/CDKN2A Antibody (CDKN2A/4499)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07384

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # CDKN2A/4499

Concentration

0.2 mg/ml

View all p16INK4a / CDKN2A Antibodies »

Product Specifications

Immunogen

Purifiedrecombinantprokaryoticfull-lengthhumanp16INK4aprotein (Uniprot: P42771)

Localization

Nuclear and cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08438)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for p16INK4a/CDKN2A Antibody (CDKN2A/4499)

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) [NBP3-07384]

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) [NBP3-07384]

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) [NBP3-07384] - Formalin-fixed, paraffin-embedded human cervix stained with p16INK4a/CDKN2A Mouse Monoclonal Antibody (CDKN2A/4499).
Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) [NBP3-07384]

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) [NBP3-07384]

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) [NBP3-07384] - Formalin-fixed, paraffin-embedded human cervix stained with p16INK4a/CDKN2A Mouse Monoclonal Antibody (CDKN2A/4499).
p16INK4a/CDKN2A Antibody (CDKN2A/4499)

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) [NBP3-07384] -

Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody (CDKN2A/4499) [NBP3-07384] - p16-positive head and neck squamous cell carcinoma (HNSCC) stained for P16INK4a. Image from verified customer review

Applications for p16INK4a/CDKN2A Antibody (CDKN2A/4499)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: p16INK4a / CDKN2A

CDKN2A/p16INK4a is a gene that codes for a widely expressed protein with four isoforms, measuring 156, 105, 116, and 167 amino acids in length with weights of approximately 17, 11, 12, and 18 kDa respectively. CDKN2A/p16INK4a functions as a negative regulator of the proliferation of cells, which then allows them to interact with cyclins D and to phosphorylate the retinoblastoma protein. Current studies are being done on several diseases and disorders related to this gene including Li-Fraumeni syndrome, malignant peripheral nerve sheath tumor, blastic plasmacytoid dendritic cell, acth-secreting pituitary adenoma, recessive dystrophic epidermolysis bullosa, marginal zone b-cell lymphoma, adult astrocytic tumor, melanoma, and squamous cell carcinoma. CDKN2A/p16INK4a has also been shown to have interactions with MDM2, ZNF420, EGR1, MYCN, and CDK6 in pathways such as the cell cycle, p53 signaling, mitochondrial apoptosis, HIF-1 Alpha, CRHR, pancreatic cancer, and glioma pathways.

Long Name

p16 Cyclin Dependent Kinase 4 Inhibitor 2A

Alternate Names

ARF, CDK4I, CDKN2A, INK4a, MLM, p14ARF, p16, p19ARF

Gene Symbol

CDKN2A

UniProt

P42771

Additional p16INK4a / CDKN2A Products

Product Documents for p16INK4a/CDKN2A Antibody (CDKN2A/4499)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p16INK4a/CDKN2A Antibody (CDKN2A/4499)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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