p16INK4a/CDKN2A Antibody [Janelia Fluor® 525]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06986JF525

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP3-06986AF350

Alexa Fluor 405

NBP3-06986AF405

Alexa Fluor 488

NBP3-06986AF488

Alexa Fluor 532

NBP3-06986AF532

Alexa Fluor 594

NBP3-06986AF594

Alexa Fluor 647

NBP3-06986AF647

Alexa Fluor 700

NBP3-06986AF700

Alexa Fluor 750

NBP3-06986AF750

Biotin

NBP3-06986B

CoraFluor 1

NBP3-06986CL1

DyLight 350

NBP3-06986UV

DyLight 405

NBP3-06986V

DyLight 488

NBP3-06986G

DyLight 550

NBP3-06986R

DyLight 594

NBP3-06986DL594

DyLight 650

NBP3-06986C

DyLight 680

NBP3-06986FR

DyLight 755

NBP3-06986IR

FITC

NBP3-06986F

HRP

NBP3-06986H

Janelia Fluor 549

NBP3-06986JF549

Janelia Fluor 646

NBP3-06986JF646

Janelia Fluor 669

NBP3-06986JF669

mFluor Violet 450 SE

NBP3-06986MFV450

mFluor Violet 500 SE

NBP3-06986MFV500

mFluor Violet 610 SE

NBP3-06986MFV610

PerCP

NBP3-06986PCP

Forumulation

Catalog #

BSA Free

NBP3-06986

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Janelia Fluor 525

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p16INK4a / CDKN2A Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human CDKN2A. The immunogen is located within the last 50 amino acids of CDKN2A.

Specificity

Human CDKN2A has 6 isoforms, including isoform 1 (156aa, 17kD), isoform 2 (105aa, 11kD), isoform 3 (116aa, 12kD), isoform 5 (167aa, 18kD), isoform tumor suppressor ARF (132aa, 14kD) and isoform smARF (85aa, 9kD). Mouse CDKN2A has 4 isoforms, including isoform 1 (168aa, 18kD), isoform 2 (125aa, 14kD), isoform tumor suppressor ARF (169aa, 19kD) and isoform smARF (125aa, 14kD). Rat CDKN2A has two isoforms, including isoform 1 (159aa, 17kD) and isoform ARF (160aa, 17kD). NBP3-06986 can detect only human isoforms 1 and 5, but cannot detect mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for p16INK4a/CDKN2A Antibody [Janelia Fluor® 525]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: p16INK4a / CDKN2A

CDKN2A/p16INK4a is a gene that codes for a widely expressed protein with four isoforms, measuring 156, 105, 116, and 167 amino acids in length with weights of approximately 17, 11, 12, and 18 kDa respectively. CDKN2A/p16INK4a functions as a negative regulator of the proliferation of cells, which then allows them to interact with cyclins D and to phosphorylate the retinoblastoma protein. Current studies are being done on several diseases and disorders related to this gene including Li-Fraumeni syndrome, malignant peripheral nerve sheath tumor, blastic plasmacytoid dendritic cell, acth-secreting pituitary adenoma, recessive dystrophic epidermolysis bullosa, marginal zone b-cell lymphoma, adult astrocytic tumor, melanoma, and squamous cell carcinoma. CDKN2A/p16INK4a has also been shown to have interactions with MDM2, ZNF420, EGR1, MYCN, and CDK6 in pathways such as the cell cycle, p53 signaling, mitochondrial apoptosis, HIF-1 Alpha, CRHR, pancreatic cancer, and glioma pathways.

Long Name

p16 Cyclin Dependent Kinase 4 Inhibitor 2A

Alternate Names

ARF, CDK4I, CDKN2A, INK4a, MLM, p14ARF, p16, p19ARF

Gene Symbol

CDKN2A

Additional p16INK4a / CDKN2A Products

Product Documents for p16INK4a/CDKN2A Antibody [Janelia Fluor® 525]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for p16INK4a/CDKN2A Antibody [Janelia Fluor® 525]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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