p19ARF/CDKN2A Antibody (5-C3-1) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB200-174B

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB200-174AF350

Alexa Fluor 405

NB200-174AF405

Alexa Fluor 488

NB200-174AF488

Alexa Fluor 532

NB200-174AF532

Alexa Fluor 594

NB200-174AF594

Alexa Fluor 647

NB200-174AF647

Alexa Fluor 700

NB200-174AF700

Alexa Fluor 750

NB200-174AF750

Allophycocyanin

NB200-174APC

Allophycocyanin/Cy7

NB200-174APCCY7

CoraFluor 1

NB200-174CL1

DyLight 350

NB200-174UV

DyLight 405

NB200-174V

DyLight 488

NB200-174G

DyLight 550

NB200-174R

DyLight 594

NB200-174DL594

DyLight 650

NB200-174C

DyLight 680

NB200-174FR

DyLight 755

NB200-174IR

FITC

NB200-174F

HRP

NB200-174H

Janelia Fluor 525

NB200-174JF525

Janelia Fluor 549

NB200-174JF549

Janelia Fluor 646

NB200-174JF646

Janelia Fluor 669

NB200-174JF669

mFluor Violet 450 SE

NB200-174MFV450

mFluor Violet 500 SE

NB200-174MFV500

mFluor Violet 610 SE

NB200-174MFV610

PE

NB200-174PE

PE/Atto594

NB200-174PEATT594

PE/Cy5.5

NB200-174PECY55

PE/Cy7

NB200-174PECY7

PerCP

NB200-174PCP

Forumulation

Catalog #

BSA Free

NB200-174

Azide and BSA Free

NBP2-80897

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Golden Syrian Hamster (Negative)

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Biotin

Antibody Source

Monoclonal Rat IgG_2B Clone # 5-C3-1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p19ARF/CDKN2A Antibodies »

Product Specifications

Immunogen

A synthetic peptide containing amino acids 54-75 of the murine p19ARF/CDKN2A. [UniProt# Q64364]

Epitope

The epitope recognized is between amino acids 62-75 of murine p19ARF/CDKN2A.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27177758). The antibody does not react with Golden Syrian hamster. Armenian hamster has not been tested.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for p19ARF/CDKN2A Antibody (5-C3-1) [Biotin]

Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) [Biotin]

Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) [Biotin] - Conducted the antibody trial with two different dilutions of p19ARF/CDKN2A Antibody (5-C3-1) [PE] (Catalog # NB200-174PE) (1:500 and 1:100) and compared them to an IgG control which was also PE labelled. Image from a verified customer review.

Applications for p19ARF/CDKN2A Antibody (5-C3-1) [Biotin]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: p19ARF/CDKN2A

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

Alternate Names

alternative reading frame, ARF, ARF-INK4a, CDK4I, CDKN2A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4), cyclin-dependent kinase inhibitor 2A, isoform 3, cyclin-dependent kinase inhibitor 2A, isoforms 1/2, cyclin-dependent kinase inhibitor protein, INK4, INK4a, Ink4a/Arf, INK4a-ARF, mitochondrial smARF', MTS1, p14, p14ARF, P16, p16(INK4a), p16-INK4, P16INK4A, p16-INK4a, P19, p19, p19Arf, Pctr1

Gene Symbol

Cdkn2a

Additional p19ARF/CDKN2A Products

Product Documents for p19ARF/CDKN2A Antibody (5-C3-1) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for p19ARF/CDKN2A Antibody (5-C3-1) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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