p21/CIP1/CDKN1A Antibody (1032)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43697

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1032

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p21/CIP1/CDKN1A Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21/CIP1/CDKN1A. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p21/CIP1/CDKN1A Antibody (1032)

Knockout Validated: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Knockout Validated: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Knockout Validated: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of:
A. 30 ug HCT116 whole cell lysate/extract (untreated)
B. 30 ug HCT116 whole cell lysate/extract (30 uM Cisplatin treatment for 24 hr)
C. 30 ug HCT116 whole cell lysate/extract (30 uM Cisplatin treatment for 48 hr) 15 % SDS-PAGE p21 Cip1 antibody [1032].
Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.
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Applications for p21/CIP1/CDKN1A Antibody (1032)

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p21/CIP1/CDKN1A

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

Additional p21/CIP1/CDKN1A Products

Product Documents for p21/CIP1/CDKN1A Antibody (1032)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p21/CIP1/CDKN1A Antibody (1032)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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