P2X4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27567
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-ETDSVVSSVTTKAK corresponding to internal region according to NP_035156.2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for P2X4 Antibody
![Western Blot: P2X4 Antibody [NBP2-27567]](https://resources.bio-techne.com/images/products/P2X4-Antibody-Western-Blot-NBP2-27567-img0004.jpg "Western Blot: P2X4 Antibody [NBP2-27567]")
Western Blot: P2X4 Antibody [NBP2-27567]
Western Blot: P2X4 Antibody [NBP2-27567] - Staining of A549 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: P2X4 Antibody [NBP2-27567]](https://resources.bio-techne.com/images/products/P2X4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27567-img0003.jpg "Immunocytochemistry/ Immunofluorescence: P2X4 Antibody [NBP2-27567]")
Immunocytochemistry/ Immunofluorescence: P2X4 Antibody [NBP2-27567]
Immunocytochemistry/Immunofluorescence: P2X4 Antibody [NBP2-27567] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).![Immunohistochemistry-Paraffin: P2X4 Antibody [NBP2-27567]](https://resources.bio-techne.com/images/products/P2X4-Antibody-Immunohistochemistry-Paraffin-NBP2-27567-img0005.jpg "Immunohistochemistry-Paraffin: P2X4 Antibody [NBP2-27567]")
Immunohistochemistry-Paraffin: P2X4 Antibody [NBP2-27567]
Immunohistochemistry-Paraffin: P2X4 Antibody [NBP2-27567] - Staining of paraffin embedded Human Uterus. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for P2X4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Immunohistochemistry-Paraffin
5 ug/mL
Peptide ELISA
Detection limit 1:16000
Western Blot
0.3 - 1 ug/mL
Application Notes
Western blot: Approx 48-50 kDa band observed in lysates of cell line A549 (calculated MW of 43.4 kDa according to Human NP_002551.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Kidney and Liver for this particular batch.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: P2X4
Long Name
P2X Purinergic Receptor 4
Alternate Names
P2RX4
Gene Symbol
P2RX4
UniProt
Additional P2X4 Products
Product Documents for P2X4 Antibody
Product Specific Notices for P2X4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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