P2X7/P2RX7 Antibody (Hano43) [PE/Cy7]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40894PECY7
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # Hano43
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A P2X7-expression construct and a final boost with P2X7-transfected HEK cells
Reactivity Notes
Reacts with Mouse.
Specificity
This antibody recognises the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for P2X7/P2RX7 Antibody (Hano43) [PE/Cy7]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: P2X7/P2RX7
Long Name
P2X Purinergic Receptor 7
Alternate Names
P2RX7
Gene Symbol
P2RX7
Additional P2X7/P2RX7 Products
Product Documents for P2X7/P2RX7 Antibody (Hano43) [PE/Cy7]
Product Specific Notices for P2X7/P2RX7 Antibody (Hano43) [PE/Cy7]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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