P2X7/P2RX7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20180
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide from mouse P2X7 conjugated to blue carrier protein was used as the antigen.
Reactivity Notes
Marmoset (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for P2X7/P2RX7 Antibody
Western Blot: P2X7/P2RX7 Antibody [NBP1-20180]
Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated at 4C overnight.Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180]
Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen.Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris HematoxylinWestern Blot: P2X7/P2RX7 Antibody [NBP1-20180]
Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - Rat brain lysate using Rabbit antibody to P2RX7 at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20Applications for P2X7/P2RX7 Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size (0.025ml) is provided in reconstituted format.
Formulation
Lyophilized from whole antisera
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: P2X7/P2RX7
Long Name
P2X Purinergic Receptor 7
Alternate Names
P2RX7
Gene Symbol
P2RX7
UniProt
Additional P2X7/P2RX7 Products
Product Documents for P2X7/P2RX7 Antibody
Product Specific Notices for P2X7/P2RX7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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