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P2Y2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15060

Novus Biologicals, part of Bio-Techne
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NBP2-15060

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human P2Y2. The exact sequence is proprietary.

Reactivity Notes

Pig (82%), Bovine (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for P2Y2 Antibody

Immunoprecipitation: P2Y2 Antibody [NBP2-15060]

Immunoprecipitation: P2Y2 Antibody [NBP2-15060]

Immunoprecipitation: P2Y2 Antibody [NBP2-15060] - Immunoprecipitation of P2Y2 protein from 293T whole cell extracts using 5 ug of P2Y2 antibody [N3C3]. Western blot analysis was performed using P2Y2 antibody [N3C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
P2Y2 Antibody

Western Blot: P2Y2 Antibody [NBP2-15060] -

Western Blot: P2Y2 Antibody [NBP2-15060] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
P2Y2 Antibody

Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] -

Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] - P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: P2Y2 stained by P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for P2Y2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: P2Y2/P2RY2

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to both adenosine and uridine nucleotides. It may participate in control of the cell cycle of endometrial carcinoma cells. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Long Name

P2Y Purinergic Receptor 2

Alternate Names

P2RU1, P2RY2, P2U, P2U1, P2Y2R

Gene Symbol

P2RY2

UniProt

Additional P2Y2/P2RY2 Products

Product Documents for P2Y2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for P2Y2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.