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P2Y4/P2RY4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14439

Novus Biologicals, part of Bio-Techne
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NBP3-14439

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Monkey (94%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from internal region of human P2Y4/P2RY4.

Epitope

Internal

Reactivity Notes

Expected reactivity based on percent sequence identity: Bovine, Opossum (88%), Mouse, Rat, Bat, Porcine (81%)

Specificity

Human P2Y4/P2RY4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for P2Y4/P2RY4 Antibody

Immunohistochemistry-Paraffin: P2Y4/P2RY4 Antibody [NBP3-14439]

Immunohistochemistry-Paraffin: P2Y4/P2RY4 Antibody [NBP3-14439]

Immunohistochemistry-Paraffin: P2Y4/P2RY4 Antibody [NBP3-14439] - Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreas after heat-induced antigen retrieval.

Applications for P2Y4/P2RY4 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: P2Y4/P2RY4

The pyrimidinergic receptor P2Y4 (P2RY4) is a member of the Purinergic Receptor subfamily. Membrane-bound P2 receptors mediate the actions of extracellular nucleotides in cell-to-cell signaling. P2Y4 expression has been documented in blood, bone, heart, lung, pancreas, placenta, and umbilical cord.

Long Name

P2Y Purinergic Receptor 4

Alternate Names

NRU, P2P, P2RY4

Gene Symbol

P2RY4

Additional P2Y4/P2RY4 Products

Product Documents for P2Y4/P2RY4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for P2Y4/P2RY4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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