p400 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB200-210
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Bovine (100%), Chimpanzee (100%), Equine (100%), Guinea Pig (100%), Monkey (100%), Porcine (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 3075 and the C-terminus (residue 3124) of human E1A binding protein p400 using the numbering given in entry AAK97789.1 (GeneID 57634).
Reactivity Notes
Gorilla (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for p400 Antibody
![Western Blot: p400 Antibody [NB200-210]](https://resources.bio-techne.com/images/products/p400-Antibody-Western-Blot-NB200-210-img0012.jpg "Western Blot: p400 Antibody [NB200-210]")
Western Blot: p400 Antibody [NB200-210]
Western Blot: p400 Antibody [NB200-210] - Detection of mouse p400 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Western Blot: p400 Antibody [NB200-210]](https://resources.bio-techne.com/images/products/p400-Antibody-Western-Blot-NB200-210-img0011.jpg "Western Blot: p400 Antibody [NB200-210]")
Western Blot: p400 Antibody [NB200-210]
Western Blot: p400 Antibody [NB200-210] - Detection of human p400 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: p400 Antibody [NB200-210]](https://resources.bio-techne.com/images/products/p400-Antibody-Immunoprecipitation-NB200-210-img0013.jpg "Immunoprecipitation: p400 Antibody [NB200-210]")
Immunoprecipitation: p400 Antibody [NB200-210]
Immunoprecipitation: p400 Antibody [NB200-210] - Detection of human p400 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p400 antibody NB200-210 (lot 2) used for IP at 6 ug per reaction. p400 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-p400 antibody from Company B. For blotting immunoprecipitated p400, NB200-210 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for p400 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:5000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: p400
Alternate Names
CAG repeat protein 32, CAGH32hDomino, DKFZP434I225, Domino homolog, E1A binding protein p400, E1A-binding protein p400, EC 3.6.1, EC 3.6.4.-, FLJ45115, KIAA1498p400 kDa SWI2/SNF2-related protein, KIAA1818, P400, p400 SWI2/SNF2-related protein, TNRC12FLJ42018, trinucleotide repeat containing 12, Trinucleotide repeat-containing gene 12 protein
Entrez Gene IDs
57634 (Human)
Gene Symbol
EP400
UniProt
Additional p400 Products
Product Documents for p400 Antibody
Product Specific Notices for p400 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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