p400 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19663

Novus Biologicals, part of Bio-Techne
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NBP2-19663
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p400 Antibodies »

Product Summary for p400 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human p400. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (83%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

343 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p400 Antibody

Western Blot: p400 Antibody [NBP2-19663]

Western Blot: p400 Antibody [NBP2-19663]

Western Blot: p400 Antibody [NBP2-19663] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: p400 Antibody [NBP2-19663]

Immunocytochemistry/ Immunofluorescence: p400 Antibody [NBP2-19663]

Immunocytochemistry/Immunofluorescence: p400 Antibody [NBP2-19663] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:200 dilution.

Applications for p400 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p400

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity.

Alternate Names

CAG repeat protein 32, CAGH32hDomino, DKFZP434I225, Domino homolog, E1A binding protein p400, E1A-binding protein p400, EC 3.6.1, EC 3.6.4.-, FLJ45115, KIAA1498p400 kDa SWI2/SNF2-related protein, KIAA1818, P400, p400 SWI2/SNF2-related protein, TNRC12FLJ42018, trinucleotide repeat containing 12, Trinucleotide repeat-containing gene 12 protein

Gene Symbol

EP400

UniProt

Q96L91 (Human)

Additional p400 Products

Product Documents for p400 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for p400 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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