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p53R2 Antibody (HL1400) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25588

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25588

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1400

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for p53R2 Antibody (HL1400) - Azide and BSA Free

Immunogen

Recombinant protein encompassing a sequence within the center region of human p53R2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (82%), Bovine (83%), Xenopus (84%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p53R2 Antibody (HL1400) - Azide and BSA Free

p53R2 Antibody (HL1400) - Azide and BSA Free

Immunohistochemistry-Paraffin: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] -

Immunohistochemistry-Paraffin: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - p53R2 antibody [HL1400] detects p53R2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. p53R2 stained by p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
p53R2 Antibody (HL1400) - Azide and BSA Free

Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] -

Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
p53R2 Antibody (HL1400) - Azide and BSA Free

Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] -

Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for p53R2 Antibody (HL1400) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53R2

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and g-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression. p53R2 is an important target of p53 for tumour suppression.

Alternate Names

RRM2B

Gene Symbol

RRM2B

Additional p53R2 Products

Product Documents for p53R2 Antibody (HL1400) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for p53R2 Antibody (HL1400) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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