p66 beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87358

Novus Biologicals, part of Bio-Techne
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NBP1-87358
NBP1-87358-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all p66 beta Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NLEVPHELPTKQDGSGVKGYEEKLNGNLRPHGDNRTAGRPGKENINDEPVDMSARRSEPERGRLTPSPDIIVLSDNEASSPRSSSRMEERLKAANLEMFKGKGIEERQQLIKQLRDELRLEEARLV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p66 beta Antibody

Immunohistochemistry-Paraffin: p66 beta Antibody [NBP1-87358]

Immunohistochemistry-Paraffin: p66 beta Antibody [NBP1-87358]

Immunohistochemistry-Paraffin: p66 beta Antibody [NBP1-87358] - Analysis in human cerebral cortex and liver tissues using NBP1-87358 antibody. Corresponding GATAD2B RNA-seq data are presented for the same tissues.
Western Blot: p66 beta Antibody [NBP1-87358]

Western Blot: p66 beta Antibody [NBP1-87358]

Western Blot: p66 beta Antibody [NBP1-87358] - Analysis in SK-BR-3 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GATAD2B antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: p66 beta Antibody [NBP1-87358]

Immunocytochemistry/ Immunofluorescence: p66 beta Antibody [NBP1-87358]

Immunocytochemistry/Immunofluorescence: p66 beta Antibody [NBP1-87358] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
View 5 more images

Applications for p66 beta Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p66 beta

P66beta/GATAD2B interacts with MBD2 (methyl-CpG-binding domain 2) and MBD3 (methyl-CpG-binding domain 3) and participates in transcriptional repression in association with the MBD2/NuRD and MBD3/NuRD histone deacetylase complexes. P66beta/GATAD2B is SUMOylated in vivo and its repressive activity has been shown to be enhanced by SUMOylation. Alternate names for P66beta/GATAD2B include transcriptional repressor p66 beta, p66/p68, GATA zinc-finger domain-containing protein 2B, FLJ37346, and KIAA1150.

Alternate Names

FLJ37346, GATA zinc finger domain containing 2B, GATA zinc finger domain-containing protein 2B, KIAA1150, MGC138257, MGC138285, p66/p68, P66beta, RP11-216N14.6, transcription repressor p66 beta component of the MeCP1 complex, transcriptional repressor p66-beta

Entrez Gene IDs

57459 (Human)

Gene Symbol

GATAD2B

UniProt

Q8WXI9 (Human)

Additional p66 beta Products

Product Documents for p66 beta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for p66 beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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