p97/VCP Antibody (Hs-14)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-455

Novus Biologicals, part of Bio-Techne
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NB500-455
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Conjugate
Catalog #
FITC
NB500-455F
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # Hs-14

Concentration

1.0 mg/ml

View all VCP Antibodies »

Product Specifications

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p97/VCP Antibody (Hs-14)

Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (Hs-14) [NB500-455]

Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (Hs-14) [NB500-455]

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (Hs-14) [NB500-455] - Intra Acrosomal Protein Antibody (Hs-14) [NB500-455] - Analysis of VCP in acetone-permeabilized human sperms using monoclonal antibody Hs-14 demonstrates its location to the acrosome. (Normal spermiogram shown).
p97/VCP Antibody (Hs-14) Flow Cytometry: p97/VCP Antibody (Hs-14) [NB500-455]

Flow Cytometry: p97/VCP Antibody (Hs-14) [NB500-455]

Separation of human sperm cells stained using anti-VCP (Hs-14) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from human sperm cells unstained by primary antibody (GAM APC, black-dashed) in Analysis (intracellular staining).

Applications for p97/VCP Antibody (Hs-14)

Application
Recommended Usage

Flow Cytometry

3-12 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

1:1000
Application Notes
Staining technique: Membrane permeabilization (acetone) is essential. Application note: The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

TBS (pH 8.0)

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VCP

This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The gene encoding this protein consists of 4 exons and its alternative splicing generates 11 distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining 7 transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.

Long Name

Valosin-containing Protein

Alternate Names

IBMPFD, TERA

Entrez Gene IDs

56 (Human); 11451 (Mouse)

Gene Symbol

VCP

Additional VCP Products

Product Documents for p97/VCP Antibody (Hs-14)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for p97/VCP Antibody (Hs-14)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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