Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 12H3

Format

BSA Free

Concentration

1 mg/ml

View all PA28 Activator alpha Subunit Antibodies »

Product Summary for PA28 Activator alpha Subunit/PSME1 Antibody (12H3) - BSA Free

Immunogen

Recombinant human PA28 Activator alpha Subunit/PSME1 (1-249aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PA28 Activator alpha Subunit/PSME1 Antibody (12H3) - BSA Free

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (12H3)BSA Free [NBP2-59434]

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (12H3)BSA Free [NBP2-59434]

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (12H3) [NBP2-59434] - Lane 1: HeLa cell lysates, Lane 2: LnCaP cell lysates, Lane 3: A549 cell lysates, Lane 4: 293T cell lysates, Lane 5: Jurkat cell lysates, Lane 6: Raji cell lysates, Lane 7: A431 cell lysates

Applications for PA28 Activator alpha Subunit/PSME1 Antibody (12H3) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PA28 Activator alpha Subunit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.

Long Name

Proteasome (Prosome, Macropain) Activator Subunit 1

Alternate Names

11S Regulator Complex Subunit alpha, IFI5111, PSME1, REG alpha

Gene Symbol

PSME1

Additional PA28 Activator alpha Subunit Products

Product Documents for PA28 Activator alpha Subunit/PSME1 Antibody (12H3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PA28 Activator alpha Subunit/PSME1 Antibody (12H3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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