PA28 Activator alpha Subunit/PSME1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19991

Novus Biologicals, part of Bio-Techne
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NBP2-19991
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (96%), Mouse (94%), Porcine (98%), Rat (95%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PA28 Activator alpha Subunit Antibodies »

Product Summary for PA28 Activator alpha Subunit/PSME1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PA28 Activator alpha Subunit/PSME1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PA28 Activator alpha Subunit/PSME1 Antibody

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991]

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991]

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991]

Immunocytochemistry/ Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991]

Immunocytochemistry/Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody [NBP2-19991] - H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PSME1 protein stained by PSME1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Applications for PA28 Activator alpha Subunit/PSME1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PA28 Activator alpha Subunit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified. [provided by RefSeq]

Long Name

Proteasome (Prosome, Macropain) Activator Subunit 1

Alternate Names

11S Regulator Complex Subunit alpha, IFI5111, PSME1, REG alpha

Gene Symbol

PSME1

UniProt

Q06323 (Human)

Additional PA28 Activator alpha Subunit Products

Product Documents for PA28 Activator alpha Subunit/PSME1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PA28 Activator alpha Subunit/PSME1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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