PA28 Activator beta Subunit/PSME2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-59752

Novus Biologicals, part of Bio-Techne
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NB100-59752
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PA28 Activator beta Subunit Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NLEKIVNPKGEEKP corresponding to C-Terminus according to NP_002809.2.

Epitope

NLEKIVNPKGEEKP

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PA28 Activator beta Subunit/PSME2 Antibody

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NB100-59752]

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NB100-59752]

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody [NB100-59752] - (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PA28 Activator beta Subunit/PSME2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1 ug/ml
Application Notes
WB: Approx. 27 kDa band observed in human spleen lysates (calculated MW of 27.4 kDa band according to NP_002809.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PA28 Activator beta Subunit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13.

Long Name

Proteasome (Prosome, Macropain) Activator Subunit 2

Alternate Names

MCP Activator, PSME2

Entrez Gene IDs

5721 (Human); 19188 (Mouse); 29614 (Rat)

Gene Symbol

PSME2

UniProt

NP_002809.2 (Human)

Additional PA28 Activator beta Subunit Products

Product Documents for PA28 Activator beta Subunit/PSME2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PA28 Activator beta Subunit/PSME2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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