PA2G4/EBP1 Antibody (9J9U5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16561
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9J9U5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 300-394 of human PA2G4/EBP1 (NP_006182.2). ELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PA2G4/EBP1 Antibody (9J9U5)
Western Blot: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561]
Western Blot: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Western blot analysis of extracts of various cell lines, using PA2G4/EBP1Rabbit mAb (NBP3-16561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Immunocytochemistry/ Immunofluorescence: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561]
Immunocytochemistry/Immunofluorescence: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Immunofluorescence analysis of NIH-3T3 cells using PA2G4/EBP1Rabbit mAb (NBP3-16561) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561]
Immunohistochemistry-Paraffin: PA2G4/EBP1 Antibody (9J9U5) [NBP3-16561] - Immunohistochemistry of paraffin-embedded mouse kidney using PA2G4/EBP1Rabbit mAb (NBP3-16561) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for PA2G4/EBP1 Antibody (9J9U5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PA2G4
Long Name
Proliferation-Associated 2G4
Alternate Names
EBP1, HG4-1
Gene Symbol
PA2G4
Additional PA2G4 Products
Product Documents for PA2G4/EBP1 Antibody (9J9U5)
Product Specific Notices for PA2G4/EBP1 Antibody (9J9U5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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