PABPN1 Antibody (6C3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26444
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6C3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PABPN1 Antibody (6C3)
Immunogen
A synthesized peptide derived from Human PABPN1 [UniProt Q86U42]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PABPN1 Antibody (6C3)
Immunohistochemistry: PABPN1 Antibody (6C3) [NBP3-26444] -
Immunohistochemistry: PABPN1 Antibody (6C3) [NBP3-26444] - Image of PABPN1 Antibody (6C3) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: PABPN1 Antibody (6C3) [NBP3-26444] -
Immunohistochemistry: PABPN1 Antibody (6C3) [NBP3-26444] - Image of PABPN1 Antibody (6C3) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: PABPN1 Antibody (6C3) [NBP3-26444] -
Western Blot: PABPN1 Antibody (6C3) [NBP3-26444] - Positive Western Blot detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate.All lanes: PABPN1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 33, 32, 38 kDa
Observed band size: 50 kDa
Applications for PABPN1 Antibody (6C3)
Application
Recommended Usage
Flow Cytometry
1:20-1:200
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PABPN1
Alternate Names
Nuclear poly(A)-binding protein 1, OPMD, PAB2poly(A) binding protein II, PABII, PABP-2, PABP2polyadenylate-binding protein 2, poly(A) binding protein 2, poly(A) binding protein, nuclear 1, Poly(A)-binding protein 2, Poly(A)-binding protein II, poly(A)-binding protein, nuclear 1, Polyadenylate-binding nuclear protein 1
Gene Symbol
PABPN1
Additional PABPN1 Products
Product Documents for PABPN1 Antibody (6C3)
Product Specific Notices for PABPN1 Antibody (6C3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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