PABPN1 Antibody (7V4X8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15539

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15539-100ul
NBP3-15539-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7V4X8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PABPN1 Antibodies »

Product Summary for PABPN1 Antibody (7V4X8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PABPN1 (Q86U42). MAAAAAAAAAAGAAGGRGSGPGRRRHLVPGAGGEAGEGAPGGAGDYGNGLESEELEPEELLLEPEPEPEPEEEPPRPRAPPGAPGPGPGSGAPGSQEEEE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PABPN1 Antibody (7V4X8)

Western Blot: PABPN1 Antibody (7V4X8) [NBP3-15539]

Western Blot: PABPN1 Antibody (7V4X8) [NBP3-15539]

Western Blot: PABPN1 Antibody (7V4X8) [NBP3-15539] - Western blot analysis of extracts of various cell lines, using PABPN1 Rabbit mAb (NBP3-15539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: PABPN1 Antibody (7V4X8) [NBP3-15539]

Immunocytochemistry/ Immunofluorescence: PABPN1 Antibody (7V4X8) [NBP3-15539]

Immunocytochemistry/Immunofluorescence: PABPN1 Antibody (7V4X8) [NBP3-15539] - Immunofluorescence analysis of U-2 OS cells using PABPN1 Rabbit mAb (NBP3-15539) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PABPN1 Antibody (7V4X8) [NBP3-15539]

Immunohistochemistry-Paraffin: PABPN1 Antibody (7V4X8) [NBP3-15539]

Immunohistochemistry-Paraffin: PABPN1 Antibody (7V4X8) [NBP3-15539] - Immunohistochemistry of paraffin-embedded mouse testis using PABPN1 Rabbit mAb (NBP3-15539) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for PABPN1 Antibody (7V4X8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PABPN1

The PABPN1 gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails on the 3' ends of eukaryotic genes and controls the size of the pol

Alternate Names

Nuclear poly(A)-binding protein 1, OPMD, PAB2poly(A) binding protein II, PABII, PABP-2, PABP2polyadenylate-binding protein 2, poly(A) binding protein 2, poly(A) binding protein, nuclear 1, Poly(A)-binding protein 2, Poly(A)-binding protein II, poly(A)-binding protein, nuclear 1, Polyadenylate-binding nuclear protein 1

Gene Symbol

PABPN1

Additional PABPN1 Products

Product Documents for PABPN1 Antibody (7V4X8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PABPN1 Antibody (7V4X8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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