PAC1R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47340

Novus Biologicals, part of Bio-Techne
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NBP2-47340
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PAC1R Antibodies »

Product Summary for PAC1R Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAC1R Antibody

Immunohistochemistry-Paraffin: PAC1R Antibody [NBP2-47340]

Immunohistochemistry-Paraffin: PAC1R Antibody [NBP2-47340]

Immunohistochemistry-Paraffin: PAC1R Antibody [NBP2-47340] - Staining in human cerebral cortex and pancreas tissues using anti-ADCYAP1R1 antibody. Corresponding ADCYAP1R1 RNA-seq data are presented for the same tissues.
Western Blot: PAC1R Antibody [NBP2-47340]

Western Blot: PAC1R Antibody [NBP2-47340]

Western Blot: PAC1R Antibody [NBP2-47340] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP2-47340]

Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP2-47340]

Immunocytochemistry/Immunofluorescence: PAC1R Antibody [NBP2-47340] - Staining of human cell line SH-SY5Y shows localization to vesicles. Antibody staining is shown in green.
View 2 more images

Applications for PAC1R Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAC1R

The pituitary adenylate cyclase-activating peptide receptor (PAC1) is a member of a family of three G-protein coupled receptors which mediate VIP (vasoactive intestinal peptide) and PACAP (pituitary adenlyate cyclase activating peptide) function in a variety of tissues. PAC1 demonstrates a higher affinity for PACAP than VIP. Expression of the VIP and VACAP receptors has been linked to the presence of human tumors.

Long Name

Pituitary Adenylate Cyclase Activating Polypeptide 1 Receptor Type I

Alternate Names

ADCYAP1R1, PACAPR, PACAPRI

Gene Symbol

ADCYAP1R1

Additional PAC1R Products

Product Documents for PAC1R Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PAC1R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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