PACSIN3 Antibody (OTI4F8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73221

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4F8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PACSIN3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PACSIN3 (NP_057307) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PACSIN3 Antibody (OTI4F8) - Azide and BSA Free

Western Blot: PACSIN3 Antibody (OTI4F8)Azide and BSA Free [NBP2-73221]

Western Blot: PACSIN3 Antibody (OTI4F8)Azide and BSA Free [NBP2-73221]

Western Blot: PACSIN3 Antibody (OTI4F8) - Azide and BSA Free [NBP2-73221] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3.
Immunohistochemistry: PACSIN3 Antibody (OTI4F8) - Azide and BSA Free [NBP2-73221]

Immunohistochemistry: PACSIN3 Antibody (OTI4F8) - Azide and BSA Free [NBP2-73221]

Immunohistochemistry: PACSIN3 Antibody (OTI4F8) - Azide and BSA Free [NBP2-73221] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PACSIN3 mouse monoclonal antibody.
Flow Cytometry: PACSIN3 Antibody (OTI4F8) - Azide and BSA Free [NBP2-73221]

Flow Cytometry: PACSIN3 Antibody (OTI4F8) - Azide and BSA Free [NBP2-73221]

Flow Cytometry: PACSIN3 Antibody (OTI4F8) - Azide and BSA Free [NBP2-73221] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PACSIN3 antibody, and then analyzed by flow cytometry.
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Applications for PACSIN3 Antibody (OTI4F8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PACSIN3

PACSIN3 (protein kinase C and casein kinase substrate in neurons protein 3) is a member of the PACSIN family, a group of adaptor protein that regulate vesicle trafficking via cytoskeletal alterations. PACSIN3 is primarily expressed in skeletal muscle and heart and has been shown to be specifically involved in the trafficking of GLUT 1 in adipocytes. PACSIN3 is also known as SH3 domain-containing 6511, syndapin III, and SDPIII.

Alternate Names

Endophilin I, FLJ17861, protein kinase C and casein kinase substrate in neurons 3, protein kinase C and casein kinase substrate in neurons protein 3, SDPIII, SH3 domain-containing protein 6511, syndapin III

Gene Symbol

PACSIN3

Additional PACSIN3 Products

Product Documents for PACSIN3 Antibody (OTI4F8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PACSIN3 Antibody (OTI4F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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